Announcing a new article publication for Zoonoses journal. Dengue viruses (DENV) and Zika virus (ZIKV) are transmitted among humans, or from non-human primates to humans, through mosquito bites. The interaction of the virus with mosquito cells is a key step in the viral life cycle. The objective of this research is to determine how DENV and ZIKV interact with mosquito cells.
Immunofluorescence assays and a direct visualization system were combined to monitor the syncytial or congregative effects of DENV and ZIKV strains on C6/36 cells. The authors of this article examine the cytopathic effects of the strains on C6/36 mosquito cells, a widely used laboratory model for studying infection with these viruses.
The results indicate that all strains of DENV-1 and DENV-2, most DENV-4 strains, and some DENV-3 strains caused syncytial effects on C6/36 cells, whereas some DENV-3 and DENV-4 strains, and all tested ZIKV strains, caused cell congregation after infection but no cell fusion. In addition, a range of environmental pH values from 6.0 to 8.0 were detected supporting virus-induced cell fusion. The optimal pH condition was 7.5, at which viral production was also highest. Furthermore, the UV-inactivated virus did not cause cell fusion, thus suggesting that viral replication may be required for DENV’s syncytial effects on C6/36 cells.
Syncytial and congregative effects of DENV and ZIKV on Aedes albopictus cells differ among viral strains. Syncytial effects of DENV on C6/36 are important for viral replication.
Zoonoses is fully open access journal for research scientists, physicians, veterinarians, and public health professionals working on diverse disciplinaries of zoonotic diseases.
Zoonoses is now open for submissions; articles can be submitted online at https://mc04.manuscriptcentral.com/zoonoses
Please visit https://zoonoses-journal.org/ to learn more about the journal.
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Submissions may be made using ScholarOne (https://mc04.manuscriptcentral.com/zoonoses).
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Dongxiao Liu, Lilian Akello Obwolo and Ruth Cruz-Cosme et al. Syncytial and Congregative Effects of Dengue and Zika Viruses on the Aedes Albopictus Cell Line Differ among Viral Strains. Zoonoses. Vol. 3(1). DOI: 10.15212/ZOONOSES-2023-0002