{"id":746,"date":"2022-09-05T16:24:25","date_gmt":"2022-09-05T16:24:25","guid":{"rendered":"https:\/\/zoonoses-journal.org\/?p=746"},"modified":"2022-09-05T16:24:25","modified_gmt":"2022-09-05T16:24:25","slug":"genetic-manipulation-toolkits-in-apicomplexan-parasites","status":"publish","type":"post","link":"https:\/\/zoonoses-journal.org\/index.php\/2022\/09\/05\/genetic-manipulation-toolkits-in-apicomplexan-parasites\/","title":{"rendered":"Genetic Manipulation Toolkits in Apicomplexan Parasites"},"content":{"rendered":"<h5>Announcing a new article publication for <em>Zoonoses<\/em> journal. Apicomplexan parasites are a group of intracellular pathogens of great medical and veterinary importance, including\u00a0<em>Toxoplasma gondii<\/em>\u00a0and\u00a0<em>Plasmodium<\/em>, which cause toxoplasmosis and malaria, respectively. Efficient and accurate manipulation of their genomes is essential to dissect their complex biology and to design new interventions. Over the past several decades, scientists have continually optimized the methods for genetic engineering in these organisms, and tremendous progress has been made.<\/h5>\n<h5>The authors of this article review the genetic manipulation tools currently used in several apicomplexan parasites, and discuss their advantages and limitations. The widely used CRISPR\/Cas9 genome editing technique has been adapted in several apicomplexans and shown promising efficiency. In contrast, conditional gene regulation is available in only a limited number of organisms, mainly\u00a0<em>Plasmodium<\/em>\u00a0and\u00a0<em>Toxoplasma<\/em>, thus posing a research bottleneck for other parasites. Conditional gene regulation can be achieved with tools that regulate gene expression at the DNA, RNA or protein level. However, a universal tool to address all needs of conditional gene manipulation remains lacking. Understanding the scope of application is key to selecting the proper method for gene manipulation.<\/h5>\n<p><strong>Article reference: <\/strong>Zhipeng Niu, Lilan Xue and Xiaoyan Yin et al. Genetic Manipulation Toolkits in Apicomplexan Parasites.\u00a0<em>Zoonoses.\u00a0<\/em>Vol. 2(1). DOI: 10.15212\/ZOONOSES-2022-0027<\/p>\n<p>https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2022-0027<\/p>\n<p><strong>Keywords:<\/strong> Genome editing, CRISPR, Apicomplexa, conditional regulation<\/p>\n<p><em>Zoonoses<\/em> is fully open access journal for research scientists, physicians, veterinarians, and public health professionals working on diverse disciplinaries of zoonotic diseases.<\/p>\n<p><em>Zoonoses<\/em> is now open for submissions; articles can be submitted online at <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a><\/p>\n<p>Please visit <a href=\"https:\/\/zoonoses-journal.org\/%20\">https:\/\/zoonoses-journal.org\/<\/a> to learn more about the journal.<\/p>\n<p><strong>Editorial Board:<\/strong> <a href=\"https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/\">https:\/\/zoonoses-journal.org\/index.php\/editorial-board<\/a><\/p>\n<p><strong><em>Zoonoses<\/em><\/strong> is available on <a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">ScienceOpen<\/a> (<a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700<\/a>).<\/p>\n<p>Submissions may be made using <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">ScholarOne<\/a> (<a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a>).<\/p>\n<p>There are no author submission or article processing fees.<\/p>\n<p>Follow <strong><em>Zoonoses <\/em><\/strong>on Twitter <a href=\"https:\/\/twitter.com\/ZoonosesJ\">@ZoonosesJ<\/a>; <a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">Facebook<\/a> (<a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114<\/a> ) and <a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">LinkedIn<\/a> (<a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">https:\/\/www.linkedin.com\/company\/zoonoses\/<\/a>)<\/p>\n<p><strong>eISSN <\/strong>2737-7474<\/p>\n<p><strong>ISSN<\/strong> 2737-7466<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Announcing a new article publication for Zoonoses journal. Apicomplexan parasites are a group of intracellular pathogens of great medical and veterinary importance, including\u00a0Toxoplasma gondii\u00a0and\u00a0Plasmodium, which cause toxoplasmosis and malaria, respectively. Efficient and accurate manipulation of their genomes is essential to dissect their complex biology and to design new interventions. Over the past several decades, scientists [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[176,177,175,174],"class_list":["post-746","post","type-post","status-publish","format-standard","hentry","category-news-and-events","tag-apicomplexa","tag-conditional-regulation","tag-crispr","tag-genome-editing"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Genetic Manipulation Toolkits in Apicomplexan Parasites - Zoonoses Journal<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zoonoses-journal.org\/index.php\/2022\/09\/05\/genetic-manipulation-toolkits-in-apicomplexan-parasites\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Genetic Manipulation Toolkits in Apicomplexan Parasites - Zoonoses Journal\" \/>\n<meta property=\"og:description\" content=\"Announcing a new article publication for Zoonoses journal. Apicomplexan parasites are a group of intracellular pathogens of great medical and veterinary importance, including\u00a0Toxoplasma gondii\u00a0and\u00a0Plasmodium, which cause toxoplasmosis and malaria, respectively. Efficient and accurate manipulation of their genomes is essential to dissect their complex biology and to design new interventions. Over the past several decades, scientists [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/zoonoses-journal.org\/index.php\/2022\/09\/05\/genetic-manipulation-toolkits-in-apicomplexan-parasites\/\" \/>\n<meta property=\"og:site_name\" content=\"Zoonoses Journal\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\" \/>\n<meta property=\"article:published_time\" content=\"2022-09-05T16:24:25+00:00\" \/>\n<meta name=\"author\" content=\"TerNo\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:site\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"TerNo\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/09\\\/05\\\/genetic-manipulation-toolkits-in-apicomplexan-parasites\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/09\\\/05\\\/genetic-manipulation-toolkits-in-apicomplexan-parasites\\\/\"},\"author\":{\"name\":\"TerNo\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/person\\\/9a2c3e8a3ddb9f181597a77f5090d067\"},\"headline\":\"Genetic Manipulation Toolkits in Apicomplexan Parasites\",\"datePublished\":\"2022-09-05T16:24:25+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/09\\\/05\\\/genetic-manipulation-toolkits-in-apicomplexan-parasites\\\/\"},\"wordCount\":352,\"publisher\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#organization\"},\"keywords\":[\"Apicomplexa\",\"conditional regulation\",\"CRISPR\",\"Genome editing\"],\"articleSection\":[\"News and Events\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/09\\\/05\\\/genetic-manipulation-toolkits-in-apicomplexan-parasites\\\/\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/09\\\/05\\\/genetic-manipulation-toolkits-in-apicomplexan-parasites\\\/\",\"name\":\"Genetic Manipulation Toolkits in Apicomplexan Parasites - 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