{"id":681,"date":"2022-05-27T08:59:13","date_gmt":"2022-05-27T08:59:13","guid":{"rendered":"https:\/\/zoonoses-journal.org\/?p=681"},"modified":"2022-05-27T09:04:14","modified_gmt":"2022-05-27T09:04:14","slug":"rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test","status":"publish","type":"post","link":"https:\/\/zoonoses-journal.org\/index.php\/2022\/05\/27\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\/","title":{"rendered":"Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test"},"content":{"rendered":"<p>Announcing a new article publication for <em>Zoonoses<\/em> journal. <em>Streptococcus suis<\/em>\u00a0serotype 2 (SS2) is an important zoonotic pathogen causing serious disease and even death in pigs and humans. Public health events and economic losses caused by SS2 have prompted widespread concern. Because of the unavailability of vaccines, the development of rapid detection methods for timely diagnosis of SS2 infection or contaminated products, and monitoring of its prevalence in susceptible animals and populations, is required to aid in the prevention and control of SS2 infections.<\/p>\n<p>The authors of this article used several sets of primers and one probe for a recombinase polymerase amplification (RPA) assay targeting the\u00a0<em>cpsJ2<\/em>\u00a0gene were designed and synthesized. Lateral flow (LF) tests in combination with RPA were used to provide visual results. Primers with high amplification efficiency were screened, and the reaction system was optimized. Indicators of detection effectiveness were evaluated. The established method had a detection limit of 100 copies\/reaction for recognizing SS2 rather than other organisms. The sensitivity was 100%, as evaluated in infected animal samples. The detection could be completed within 20 min and required only constant temperature equipment.<\/p>\n<p>The established rapid, visual, sensitive and specific RPA-LF assay showed superior detection performance and is expected to be widely applied to fight SS2 infection in resource-limited areas.<\/p>\n<p><strong>Article reference:<\/strong> Yong Qi, Wei Li and Xiaoling Li et al. Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test.\u00a0<em>Zoonoses.\u00a0<\/em>Vol. 2(1). DOI: 10.15212\/ZOONOSES-2022-0015<\/p>\n<p>https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2022-0015<\/p>\n<p><strong>Keywords:<\/strong> Streptococcus suis serotype 2 , recombinase polymerase amplification, lateral flow test, detection, cpsJ2 gene<\/p>\n<p><em>Zoonoses<\/em> is fully open access journal for research scientists, physicians, veterinarians, and public health professionals working on diverse disciplinaries of zoonotic diseases.<\/p>\n<p><em>Zoonoses<\/em> is now open for submissions; articles can be submitted online at <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a><\/p>\n<p>Please visit <a href=\"https:\/\/zoonoses-journal.org\/%20\">https:\/\/zoonoses-journal.org\/<\/a> to learn more about the journal.<\/p>\n<p><strong>Editorial Board:<\/strong> <a href=\"https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/\">https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/<\/a><\/p>\n<p><strong><em>Zoonoses<\/em><\/strong> is available on <a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">ScienceOpen<\/a> (<a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700<\/a>).<\/p>\n<p>Submissions may be made using <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">ScholarOne<\/a> (<a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a>).<\/p>\n<p>There are no author submission or article processing fees.<\/p>\n<p>Follow <strong><em>Zoonoses <\/em><\/strong>on Twitter <a href=\"https:\/\/twitter.com\/ZoonosesJ\">@ZoonosesJ<\/a>; <a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">Facebook<\/a> (<a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114<\/a> ) and <a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">LinkedIn<\/a> (<a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">https:\/\/www.linkedin.com\/company\/zoonoses\/<\/a>)<\/p>\n<p><strong>\u00a0<\/strong><\/p>\n<p><strong>eISSN <\/strong>2737-7474<\/p>\n<p><strong>ISSN<\/strong> 2737-7466<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Announcing a new article publication for Zoonoses journal. Streptococcus suis\u00a0serotype 2 (SS2) is an important zoonotic pathogen causing serious disease and even death in pigs and humans. Public health events and economic losses caused by SS2 have prompted widespread concern. Because of the unavailability of vaccines, the development of rapid detection methods for timely diagnosis [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[129,128,127,113,126],"class_list":["post-681","post","type-post","status-publish","format-standard","hentry","category-news-and-events","tag-cpsj2-gene","tag-detection","tag-lateral-flow-test","tag-recombinase-polymerase-amplification","tag-streptococcus-suis-serotype-2"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test - Zoonoses Journal<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zoonoses-journal.org\/index.php\/2022\/05\/27\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test - Zoonoses Journal\" \/>\n<meta property=\"og:description\" content=\"Announcing a new article publication for Zoonoses journal. Streptococcus suis\u00a0serotype 2 (SS2) is an important zoonotic pathogen causing serious disease and even death in pigs and humans. Public health events and economic losses caused by SS2 have prompted widespread concern. Because of the unavailability of vaccines, the development of rapid detection methods for timely diagnosis [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/zoonoses-journal.org\/index.php\/2022\/05\/27\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\/\" \/>\n<meta property=\"og:site_name\" content=\"Zoonoses Journal\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\" \/>\n<meta property=\"article:published_time\" content=\"2022-05-27T08:59:13+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2022-05-27T09:04:14+00:00\" \/>\n<meta name=\"author\" content=\"TerNo\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:site\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"TerNo\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/05\\\/27\\\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/05\\\/27\\\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\\\/\"},\"author\":{\"name\":\"TerNo\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/person\\\/9a2c3e8a3ddb9f181597a77f5090d067\"},\"headline\":\"Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test\",\"datePublished\":\"2022-05-27T08:59:13+00:00\",\"dateModified\":\"2022-05-27T09:04:14+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/05\\\/27\\\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\\\/\"},\"wordCount\":404,\"publisher\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#organization\"},\"keywords\":[\"cpsJ2 gene\",\"detection\",\"lateral flow test\",\"recombinase polymerase amplification\",\"Streptococcus suis serotype 2\"],\"articleSection\":[\"News and Events\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/05\\\/27\\\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\\\/\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2022\\\/05\\\/27\\\/rapid-visual-detection-of-pathogenic-streptococcus-suis-type-2-through-a-recombinase-polymerase-amplification-assay-coupled-with-lateral-flow-test\\\/\",\"name\":\"Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test - 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