{"id":2410,"date":"2026-09-07T13:23:16","date_gmt":"2026-09-07T13:23:16","guid":{"rendered":"https:\/\/zoonoses-journal.org\/?p=2410"},"modified":"2026-09-07T13:24:07","modified_gmt":"2026-09-07T13:24:07","slug":"cryptosporidium-parvum-molecular-epidemiological-investigation","status":"publish","type":"post","link":"https:\/\/zoonoses-journal.org\/index.php\/2026\/09\/07\/cryptosporidium-parvum-molecular-epidemiological-investigation\/","title":{"rendered":"Molecular and Epidemiological Investigation of Cryptosporidium parvum Infections in Calves in Beni-Suef Governorate, Egypt"},"content":{"rendered":"<p>Announcing a new article publication for <em>Zoonoses<\/em> journal. This study was aimed at performing molecular and epidemiological investigation of\u00a0<em>Cryptosporidium<\/em>\u00a0spp. in calves in Beni-Suef governorate, Egypt.<\/p>\n<p>Fecal samples were collected from 350 calves 2\u201312 weeks old. Samples were microscopically screened for\u00a0<em>Cryptosporidium spp.<\/em>\u00a0through a modified Ziehl-Neelsen staining technique followed by two-step nested PCR flanking the gp60 gene.<\/p>\n<p><em>Cryptosporidium<\/em>\u00a0was macroscopically detected in 28.3% of fecal samples in younger group (2-8 weeks). The prevalence of infections was higher in diarrheic calves and during the rainy season. Most infections occurred at ages &lt;8 weeks. The gp60 gene-based PCR and sequences confirmed the presence of\u00a0<em>Cryptosporidium<\/em>\u00a0species belonging to\u00a0<em>Cryptosporidium parvum<\/em>\u00a0subtype family IIa.<\/p>\n<p>Microscopic examination aided in the initial detection, whereas molecular analysis confirmed the presence of zoonotic\u00a0<em>C. parvum<\/em>. However, the absence of comprehensive gp60 subtyping and broader molecular screening limited detailed epidemiological characterization. More extensive molecular investigations are therefore required in future studies.<\/p>\n<p>Read More: <a href=\"https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2026-0057\">https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2026-0057<\/a><\/p>\n<p><strong># # # # # #<\/strong><\/p>\n<p><em>Zoonoses<\/em> is fully open access journal for research scientists, physicians, veterinarians, and public health professionals working on diverse disciplinaries of zoonotic diseases. Please visit <a href=\"https:\/\/zoonoses-journal.org\/%20\">https:\/\/zoonoses-journal.org\/<\/a> to learn more about the journal.<\/p>\n<p><em>Zoonoses<\/em> is now open for submissions; articles can be submitted online at <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a><\/p>\n<p>There are no author submission or article processing fees.<\/p>\n<p><strong>Editorial Board:<\/strong> <a href=\"https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/\">https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/<\/a><\/p>\n<p><strong><em>Zoonoses<\/em><\/strong> is available on <a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">ScienceOpen<\/a> (<a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700<\/a>).<\/p>\n<p>Follow <strong><em>Zoonoses <\/em><\/strong>on Twitter <a href=\"https:\/\/twitter.com\/ZoonosesJ\">@ZoonosesJ<\/a>; <a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">Facebook<\/a> (<a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114<\/a> ) and <a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">LinkedIn<\/a> (<a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">https:\/\/www.linkedin.com\/company\/zoonoses\/<\/a>)<\/p>\n<p><strong>eISSN <\/strong>2737-7474<\/p>\n<p><strong>ISSN<\/strong> 2737-7466<\/p>\n<p><strong># # # # # #<\/strong><\/p>\n<p>Hanaa H. Ali, Eman H. Abdel-Rahman, Sahar A. Abdel Aziz, Shawky M. Aboelhadid. Molecular and Epidemiological Investigation of\u00a0<em>Cryptosporidium parvum<\/em>\u00a0Infections in Calves in Beni-Suef Governorate, Egypt.\u00a0<em>Zoonoses.\u00a0<\/em>2026. Vol. 6(1). DOI: \u00a010.15212\/ZOONOSES-2026-0057<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Announcing a new article publication for Zoonoses journal. This study was aimed at performing molecular and epidemiological investigation of\u00a0Cryptosporidium\u00a0spp. in calves in Beni-Suef governorate, Egypt. Fecal samples were collected from 350 calves 2\u201312 weeks old. Samples were microscopically screened for\u00a0Cryptosporidium spp.\u00a0through a modified Ziehl-Neelsen staining technique followed by two-step nested PCR flanking the gp60 gene. [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":2412,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[799,800,363,420,801],"class_list":["post-2410","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-and-events","tag-cryptosporidium-parvum","tag-gp60-subtyping","tag-pcr","tag-phylogenetic-analysis","tag-zoonotic-parasites"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Molecular and Epidemiological Investigation of Cryptosporidium parvum Infections in Calves in Beni-Suef Governorate, Egypt - Zoonoses Journal<\/title>\n<meta name=\"description\" content=\"Microscopy and molecular analysis confirmed zoonotic Cryptosporidium parvum, but limited gp60 subtyping highlights the need for expanded molecular epidemiology.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zoonoses-journal.org\/index.php\/2026\/09\/07\/cryptosporidium-parvum-molecular-epidemiological-investigation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Molecular and Epidemiological Investigation of Cryptosporidium parvum Infections in Calves in Beni-Suef Governorate, Egypt - Zoonoses Journal\" \/>\n<meta property=\"og:description\" content=\"Microscopy and molecular analysis confirmed zoonotic Cryptosporidium parvum, but limited gp60 subtyping highlights the need for expanded molecular epidemiology.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/zoonoses-journal.org\/index.php\/2026\/09\/07\/cryptosporidium-parvum-molecular-epidemiological-investigation\/\" \/>\n<meta property=\"og:site_name\" content=\"Zoonoses Journal\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\" \/>\n<meta property=\"article:published_time\" content=\"2026-09-07T13:23:16+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-09-07T13:24:07+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2026\/09\/7-9-2026-Molecular-and-Epidemiological-Investigation.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1417\" \/>\n\t<meta property=\"og:image:height\" content=\"386\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Hanaa H. Ali\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:site\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Hanaa H. Ali\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2026\\\/09\\\/07\\\/cryptosporidium-parvum-molecular-epidemiological-investigation\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2026\\\/09\\\/07\\\/cryptosporidium-parvum-molecular-epidemiological-investigation\\\/\"},\"author\":{\"name\":\"Hanaa H. 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