{"id":1222,"date":"2024-01-05T11:15:52","date_gmt":"2024-01-05T11:15:52","guid":{"rendered":"https:\/\/zoonoses-journal.org\/?p=1222"},"modified":"2024-08-22T13:13:36","modified_gmt":"2024-08-22T13:13:36","slug":"molecular-diagnosis-of-scrub-typhus-sample-and-timing-matter","status":"publish","type":"post","link":"https:\/\/zoonoses-journal.org\/index.php\/2024\/01\/05\/molecular-diagnosis-of-scrub-typhus-sample-and-timing-matter\/","title":{"rendered":"Molecular Diagnosis of Scrub Typhus: Sample and Timing Matter"},"content":{"rendered":"

Announcing a new article publication for Zoonoses<\/em> journal<\/a>. \u00a0Scrub typhus (ST) is an acute febrile illness caused by\u00a0Orientia tsutsugamushi<\/em>. Laboratory tests are needed to confirm the diagnosis when the characteristic eschar is absent. This study aimed to evaluate the performance of 47 kDa qPCR using clotted and EDTA-treated blood among patients with suspected ST<\/p>\n

Clotted blood samples from 284 inpatients (IPs) and EDTA-treated blood samples from 194 outpatients (OPs) with suspected scrub typhus were collected from patients who were blood culture- and malaria-negative. ST IgM ELISA and 47 kDa qPCR were performed for detection of ST infections.<\/p>\n

Among the IPs (n=284) and OPs (n=194), 41% and 63% were confirmed to have ST based on the case definition, respectively; the mortality rate was 1%. The 47 kDa qPCR sensitivity of the buffy coat (65.6%) was greater compared to clotted blood (37.1%). PCR was more likely to be positive in the 1st week of the illness; IgM ELISA positivity increased after the 5th day of the illness.<\/p>\n

The type of sample and the time of sample collection have a role in the diagnosis of ST. These preliminary results need to be confirmed by prospective multicentric studies.<\/p>\n

https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2023-0019<\/a><\/p>\n

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eISSN <\/strong>2737-7474<\/p>\n

ISSN<\/strong> 2737-7466<\/p>\n

Nagarajan L Surya, Sania Paul and Susmitha K Perumalla et al. Molecular Diagnosis of Scrub Typhus: Sample and Timing Matter.\u00a0Zoonoses.\u00a0<\/em>2024. Vol. 4(1). DOI: 10.15212\/ZOONOSES-2023-0019<\/p>\n","protected":false},"excerpt":{"rendered":"

Announcing a new article publication for Zoonoses journal. \u00a0Scrub typhus (ST) is an acute febrile illness caused by\u00a0Orientia tsutsugamushi. Laboratory tests are needed to confirm the diagnosis when the characteristic eschar is absent. This study aimed to evaluate the performance of 47 kDa qPCR using clotted and EDTA-treated blood among patients with suspected ST Clotted […]<\/p>\n","protected":false},"author":5,"featured_media":1446,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[365,364,366,61,363],"class_list":["post-1222","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-and-events","tag-eschar","tag-igm-elisa","tag-mortality","tag-orientia-tsutsugamushi","tag-pcr"],"yoast_head":"\nMolecular Diagnosis of Scrub Typhus: Sample and Timing Matter<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zoonoses-journal.org\/index.php\/2024\/01\/05\/molecular-diagnosis-of-scrub-typhus-sample-and-timing-matter\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Molecular Diagnosis of Scrub Typhus: Sample and Timing Matter\" \/>\n<meta property=\"og:description\" content=\"Announcing a new article publication for Zoonoses journal. \u00a0Scrub typhus (ST) is an acute febrile illness caused by\u00a0Orientia tsutsugamushi. 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