{"id":1123,"date":"2023-10-13T11:54:20","date_gmt":"2023-10-13T11:54:20","guid":{"rendered":"https:\/\/zoonoses-journal.org\/?p=1123"},"modified":"2024-08-29T13:40:58","modified_gmt":"2024-08-29T13:40:58","slug":"optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction","status":"publish","type":"post","link":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/","title":{"rendered":"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction"},"content":{"rendered":"

Announcing a new article publication for Zoonoses<\/em> journal<\/a>. Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction.<\/p>\n

The effects of different SPRI bead ratios, incubation times, and elution times were compared for three different initial sample amounts. An L9(33<\/sup>) orthogonal experiment was designed to determine the optimal combination of these factors.<\/p>\n

The incubation time of three factors including SPRI beads ratio, incubation time, and elution time had a statistically significant effect on the recovery rate for the initial sample amount of 1500 ng and 3000 ng. The orthogonal experiment results indicated that incubation time had the greatest impact among the three factors.<\/p>\n

Incubation time significantly influences recovery rate in full-length 16S rDNA high-throughput sequencing library construction. The use of 0.8\u00d7 SPRI beads, 15 minutes of incubation, and 10 minutes of elution resulted in the highest recovery rate. SPRI beads offer a viable method for recovering full-length 16S rDNA amplicons.<\/p>\n

Read More: https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2023-0007<\/a><\/p>\n

Zoonoses<\/em><\/a> is fully open access journal for research scientists, physicians, veterinarians, and public health professionals working on diverse disciplinaries of zoonotic diseases.<\/p>\n

Zoonoses<\/em> is now open for submissions; articles can be submitted online at https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a><\/p>\n

Please visit https:\/\/zoonoses-journal.org\/<\/a> to learn more about the journal.<\/p>\n

Editorial Board:<\/strong> https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/<\/a><\/p>\n

Zoonoses<\/em><\/strong> is available on ScienceOpen<\/a> (https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700<\/a>).<\/p>\n

Submissions may be made using ScholarOne<\/a> (https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a>).<\/p>\n

There are no author submission or article processing fees.<\/p>\n

Follow Zoonoses <\/em><\/strong>on Twitter @ZoonosesJ<\/a>; Facebook<\/a> (https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114<\/a> ) and LinkedIn<\/a> (https:\/\/www.linkedin.com\/company\/zoonoses\/<\/a>)<\/p>\n

eISSN <\/strong>2737-7474<\/p>\n

ISSN<\/strong> 2737-7466<\/p>\n

Yinmei Li, Ziqiang He and Mimi Kong et al. Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction.\u00a0Zoonoses.\u00a0<\/em>2023. Vol. 3(1). DOI: 10.15212\/ZOONOSES-2023-0007<\/p>\n","protected":false},"excerpt":{"rendered":"

Announcing a new article publication for Zoonoses journal. Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction. The effects […]<\/p>\n","protected":false},"author":5,"featured_media":1463,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[330,331,333,332,329],"class_list":["post-1123","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-and-events","tag-full-length-16s-rdna-high-throughput-sequencing","tag-library-construction","tag-nucleic-acid-purification-and-recovery","tag-recovery-rate","tag-spri-beads"],"yoast_head":"\nOptimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction<\/title>\n<meta name=\"description\" content=\"Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction\" \/>\n<meta property=\"og:description\" content=\"Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/\" \/>\n<meta property=\"og:site_name\" content=\"Zoonoses Journal\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\" \/>\n<meta property=\"article:published_time\" content=\"2023-10-13T11:54:20+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-08-29T13:40:58+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2023\/10\/zoonoses20230007_fig2.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1423\" \/>\n\t<meta property=\"og:image:height\" content=\"1073\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Pdrtiru\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:site\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Pdrtiru\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/\"},\"author\":{\"name\":\"Pdrtiru\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/person\\\/7b94348b9f4f5d3983636ac0a201853f\"},\"headline\":\"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction\",\"datePublished\":\"2023-10-13T11:54:20+00:00\",\"dateModified\":\"2024-08-29T13:40:58+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/\"},\"wordCount\":355,\"publisher\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2023\\\/10\\\/zoonoses20230007_fig2.jpg\",\"keywords\":[\"full-length 16S rDNA high-throughput sequencing\",\"library construction\",\"nucleic acid purification and recovery\",\"recovery rate\",\"SPRI beads\"],\"articleSection\":[\"News and Events\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/\",\"name\":\"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2023\\\/10\\\/zoonoses20230007_fig2.jpg\",\"datePublished\":\"2023-10-13T11:54:20+00:00\",\"dateModified\":\"2024-08-29T13:40:58+00:00\",\"description\":\"Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/#primaryimage\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2023\\\/10\\\/zoonoses20230007_fig2.jpg\",\"contentUrl\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2023\\\/10\\\/zoonoses20230007_fig2.jpg\",\"width\":1423,\"height\":1073,\"caption\":\"Solid-Phase Reversible Immobilization Beads\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/10\\\/13\\\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/zoonoses-journal.org\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#website\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/\",\"name\":\"Zoonoses Journal\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/zoonoses-journal.org\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#organization\",\"name\":\"Zoonoses Journal\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2021\\\/03\\\/cropped-cropped-Zoonoses_logo_small-1.png\",\"contentUrl\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2021\\\/03\\\/cropped-cropped-Zoonoses_logo_small-1.png\",\"width\":512,\"height\":512,\"caption\":\"Zoonoses Journal\"},\"image\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/Zoonoses-Journal-100462755574114\",\"https:\\\/\\\/x.com\\\/ZoonosesJ\",\"https:\\\/\\\/www.linkedin.com\\\/company\\\/78022634\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/person\\\/7b94348b9f4f5d3983636ac0a201853f\",\"name\":\"Pdrtiru\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/litespeed\\\/avatar\\\/3383aac56276a7e5bca01376d31d06d5.jpg?ver=1777997335\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/litespeed\\\/avatar\\\/3383aac56276a7e5bca01376d31d06d5.jpg?ver=1777997335\",\"contentUrl\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/litespeed\\\/avatar\\\/3383aac56276a7e5bca01376d31d06d5.jpg?ver=1777997335\",\"caption\":\"Pdrtiru\"},\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/author\\\/thiruvalluvan\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction","description":"Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/","og_locale":"en_US","og_type":"article","og_title":"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction","og_description":"Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction.","og_url":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/","og_site_name":"Zoonoses Journal","article_publisher":"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114","article_published_time":"2023-10-13T11:54:20+00:00","article_modified_time":"2024-08-29T13:40:58+00:00","og_image":[{"width":1423,"height":1073,"url":"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2023\/10\/zoonoses20230007_fig2.jpg","type":"image\/jpeg"}],"author":"Pdrtiru","twitter_card":"summary_large_image","twitter_creator":"@ZoonosesJ","twitter_site":"@ZoonosesJ","twitter_misc":{"Written by":"Pdrtiru","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/#article","isPartOf":{"@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/"},"author":{"name":"Pdrtiru","@id":"https:\/\/zoonoses-journal.org\/#\/schema\/person\/7b94348b9f4f5d3983636ac0a201853f"},"headline":"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction","datePublished":"2023-10-13T11:54:20+00:00","dateModified":"2024-08-29T13:40:58+00:00","mainEntityOfPage":{"@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/"},"wordCount":355,"publisher":{"@id":"https:\/\/zoonoses-journal.org\/#organization"},"image":{"@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/#primaryimage"},"thumbnailUrl":"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2023\/10\/zoonoses20230007_fig2.jpg","keywords":["full-length 16S rDNA high-throughput sequencing","library construction","nucleic acid purification and recovery","recovery rate","SPRI beads"],"articleSection":["News and Events"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/","url":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/","name":"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction","isPartOf":{"@id":"https:\/\/zoonoses-journal.org\/#website"},"primaryImageOfPage":{"@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/#primaryimage"},"image":{"@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/#primaryimage"},"thumbnailUrl":"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2023\/10\/zoonoses20230007_fig2.jpg","datePublished":"2023-10-13T11:54:20+00:00","dateModified":"2024-08-29T13:40:58+00:00","description":"Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This article investigates the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction.","breadcrumb":{"@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/#primaryimage","url":"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2023\/10\/zoonoses20230007_fig2.jpg","contentUrl":"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2023\/10\/zoonoses20230007_fig2.jpg","width":1423,"height":1073,"caption":"Solid-Phase Reversible Immobilization Beads"},{"@type":"BreadcrumbList","@id":"https:\/\/zoonoses-journal.org\/index.php\/2023\/10\/13\/optimizing-the-use-of-solid-phase-reversible-immobilization-beads-for-high-throughput-full-length-16s-rdna-sequencing-library-construction\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/zoonoses-journal.org\/"},{"@type":"ListItem","position":2,"name":"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction"}]},{"@type":"WebSite","@id":"https:\/\/zoonoses-journal.org\/#website","url":"https:\/\/zoonoses-journal.org\/","name":"Zoonoses Journal","description":"","publisher":{"@id":"https:\/\/zoonoses-journal.org\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/zoonoses-journal.org\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/zoonoses-journal.org\/#organization","name":"Zoonoses Journal","url":"https:\/\/zoonoses-journal.org\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/zoonoses-journal.org\/#\/schema\/logo\/image\/","url":"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2021\/03\/cropped-cropped-Zoonoses_logo_small-1.png","contentUrl":"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2021\/03\/cropped-cropped-Zoonoses_logo_small-1.png","width":512,"height":512,"caption":"Zoonoses Journal"},"image":{"@id":"https:\/\/zoonoses-journal.org\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114","https:\/\/x.com\/ZoonosesJ","https:\/\/www.linkedin.com\/company\/78022634"]},{"@type":"Person","@id":"https:\/\/zoonoses-journal.org\/#\/schema\/person\/7b94348b9f4f5d3983636ac0a201853f","name":"Pdrtiru","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/zoonoses-journal.org\/wp-content\/litespeed\/avatar\/3383aac56276a7e5bca01376d31d06d5.jpg?ver=1777997335","url":"https:\/\/zoonoses-journal.org\/wp-content\/litespeed\/avatar\/3383aac56276a7e5bca01376d31d06d5.jpg?ver=1777997335","contentUrl":"https:\/\/zoonoses-journal.org\/wp-content\/litespeed\/avatar\/3383aac56276a7e5bca01376d31d06d5.jpg?ver=1777997335","caption":"Pdrtiru"},"url":"https:\/\/zoonoses-journal.org\/index.php\/author\/thiruvalluvan\/"}]}},"_links":{"self":[{"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/posts\/1123","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/comments?post=1123"}],"version-history":[{"count":3,"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/posts\/1123\/revisions"}],"predecessor-version":[{"id":1464,"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/posts\/1123\/revisions\/1464"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/media\/1463"}],"wp:attachment":[{"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/media?parent=1123"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/categories?post=1123"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/zoonoses-journal.org\/index.php\/wp-json\/wp\/v2\/tags?post=1123"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}