{"id":1111,"date":"2023-09-25T05:32:12","date_gmt":"2023-09-25T05:32:12","guid":{"rendered":"https:\/\/zoonoses-journal.org\/?p=1111"},"modified":"2024-08-28T12:51:18","modified_gmt":"2024-08-28T12:51:18","slug":"rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification","status":"publish","type":"post","link":"https:\/\/zoonoses-journal.org\/index.php\/2023\/09\/25\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\/","title":{"rendered":"Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification"},"content":{"rendered":"<p>Announcing a new article publication for <a href=\"https:\/\/zoonoses-journal.org\/\"><em>Zoonoses<\/em> journal<\/a>. \u00a0Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. Because BV infects humans through bites, scratches, and other injuries inflicted by macaques, the simple and rapid diagnosis of BV in field laboratories is of great importance to protect veterinarians, laboratory researchers, and support personnels from the threat of infection.<\/p>\n<p>Two recombinase polymerase amplification (RPA) assays with a closed vertical flow (VF) visualization strip (RPA-VF-UL27 and RPA-VF-US6) were developed that target two conserved genes combined with a one-off, closed visualization strip device. The sensitivities and specificities of the two assays were compared after optimization of the reaction conditions. The performance of RPA-VF-US6 at room temperature was determined to evaluate its potential in point-of-care (POC) testing.<\/p>\n<p>RPA-VF-US6 specifically detected the positive plasmid control (rather than nucleic acids of herpesviruses) with a detection limit of 28 copies, while RPA-VF-UL27 had cross-reactivity with HSV-1, but even 3.4 copies of plasmid standards were readout by this assay. Moreover, RPA-VF-US6 had excellent performance at room temperature (the detection limit was 2,800 plasmid copies), indicating the potential of RPA-VF-US6 in POC testing.<\/p>\n<p>Two RPA assays for BV visualization diagnosis were developed. RPA-VF-US6 is a simple, rapid, and specific detection method for BV. The entire reaction can be performed at a constant temperature within 30 min, suggesting the potential of RPA-VF-US6 for POC testing in field laboratories without sophisticated instruments.<\/p>\n<p><a href=\"https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2023-0031\">https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2023-0031<\/a><\/p>\n<p><a href=\"https:\/\/zoonoses-journal.org\/\"><em>Zoonoses<\/em><\/a> is fully open access journal for research scientists, physicians, veterinarians, and public health professionals working on diverse disciplinaries of zoonotic diseases.<\/p>\n<p><em>Zoonoses<\/em> is now open for submissions; articles can be submitted online at <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a><\/p>\n<p>Please visit <a href=\"https:\/\/zoonoses-journal.org\/%20\">https:\/\/zoonoses-journal.org\/<\/a> to learn more about the journal.<\/p>\n<p><strong>Editorial Board:<\/strong> <a href=\"https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/\">https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/<\/a><\/p>\n<p><strong><em>Zoonoses<\/em><\/strong> is available on <a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">ScienceOpen<\/a> (<a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700<\/a>).<\/p>\n<p>Submissions may be made using <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">ScholarOne<\/a> (<a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a>).<\/p>\n<p>There are no author submission or article processing fees.<\/p>\n<p>Follow <strong><em>Zoonoses <\/em><\/strong>on Twitter <a href=\"https:\/\/twitter.com\/ZoonosesJ\">@ZoonosesJ<\/a>; <a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">Facebook<\/a> (<a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114<\/a> ) and <a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">LinkedIn<\/a> (<a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">https:\/\/www.linkedin.com\/company\/zoonoses\/<\/a>)<\/p>\n<p><strong>eISSN <\/strong>2737-7474<\/p>\n<p><strong>ISSN<\/strong> 2737-7466<\/p>\n<p>Xinlan Chen, Chenchen Liu and Fangxu Li et al. Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification.\u00a0<em>Zoonoses.\u00a0<\/em>2023. Vol. 3(1). DOI: 10.15212\/ZOONOSES-2023-0031<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Announcing a new article publication for Zoonoses journal. \u00a0Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. Because BV infects humans through bites, scratches, and other injuries inflicted by macaques, the simple and rapid diagnosis of BV in field laboratories is of great importance to protect [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":1459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[325,328,113,327,326],"class_list":["post-1111","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news-and-events","tag-bv-diagnosis","tag-poc-testing","tag-recombinase-polymerase-amplification","tag-rpa-assay","tag-visualization-diagnosis"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification<\/title>\n<meta name=\"description\" content=\"Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. Because BV infects humans through bites, scratches, and other injuries inflicted by macaques, the simple and rapid diagnosis of BV in field laboratories is of great importance to protect veterinarians, laboratory researchers, and support personnels from the threat of infection.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zoonoses-journal.org\/index.php\/2023\/09\/25\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification\" \/>\n<meta property=\"og:description\" content=\"Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. Because BV infects humans through bites, scratches, and other injuries inflicted by macaques, the simple and rapid diagnosis of BV in field laboratories is of great importance to protect veterinarians, laboratory researchers, and support personnels from the threat of infection.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/zoonoses-journal.org\/index.php\/2023\/09\/25\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\/\" \/>\n<meta property=\"og:site_name\" content=\"Zoonoses Journal\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\" \/>\n<meta property=\"article:published_time\" content=\"2023-09-25T05:32:12+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-08-28T12:51:18+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/zoonoses-journal.org\/wp-content\/uploads\/2023\/09\/Rapid-and-Visual-Detection-of-Monkey-B-Virus-Zoonoses.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1985\" \/>\n\t<meta property=\"og:image:height\" content=\"2367\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Pdrtiru\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:site\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Pdrtiru\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/\"},\"author\":{\"name\":\"Pdrtiru\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/person\\\/7b94348b9f4f5d3983636ac0a201853f\"},\"headline\":\"Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification\",\"datePublished\":\"2023-09-25T05:32:12+00:00\",\"dateModified\":\"2024-08-28T12:51:18+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/\"},\"wordCount\":401,\"publisher\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2023\\\/09\\\/Rapid-and-Visual-Detection-of-Monkey-B-Virus-Zoonoses.jpg\",\"keywords\":[\"BV diagnosis\",\"POC testing\",\"recombinase polymerase amplification\",\"RPA assay\",\"visualization diagnosis\"],\"articleSection\":[\"News and Events\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/\",\"name\":\"Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/25\\\/rapid-and-visual-detection-of-monkey-b-virus-based-on-recombinase-polymerase-amplification\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/zoonoses-journal.org\\\/wp-content\\\/uploads\\\/2023\\\/09\\\/Rapid-and-Visual-Detection-of-Monkey-B-Virus-Zoonoses.jpg\",\"datePublished\":\"2023-09-25T05:32:12+00:00\",\"dateModified\":\"2024-08-28T12:51:18+00:00\",\"description\":\"Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. 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