{"id":1104,"date":"2023-09-07T05:32:13","date_gmt":"2023-09-07T05:32:13","guid":{"rendered":"https:\/\/zoonoses-journal.org\/?p=1104"},"modified":"2023-09-07T05:32:13","modified_gmt":"2023-09-07T05:32:13","slug":"visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification","status":"publish","type":"post","link":"https:\/\/zoonoses-journal.org\/index.php\/2023\/09\/07\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\/","title":{"rendered":"Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification"},"content":{"rendered":"<p>Announcing a new article publication for <em>Zoonoses<\/em> journal. <em>Escherichia coli<\/em>\u00a0O157:H7 (<em>E. coli<\/em>\u00a0O157:H7) can induce severe diseases in animals and humans that result in significant public health problems. Therefore, the development of rapid and visual detection methods to diagnose\u00a0<em>E. coli<\/em>\u00a0O157:H7 infections and monitor its prevalence is critical for the prevention and control purposes.<\/p>\n<p>A colorimetric loop-mediated isothermal amplification (LAMP) assay was utilized to detect\u00a0<em>E. coli<\/em>\u00a0O157:H7. A DNA extraction kit and Flinders Technology Associates (FTA) cards were used to extract nucleic acid in conjunction with colorimetric LAMP detection.<\/p>\n<p>The method developed effectively distinguished\u00a0<em>E. coli<\/em>\u00a0O157:H7 from other pathogens with a detection limit of 25 CFU\/mL in spiked stool samples. In addition, the nucleic acid of these samples was easily extracted and transported with an FTA card at room temperature. The entire detection process was completed within 35 min using simple constant-temperature equipment.<\/p>\n<p>The colorimetric LAMP method with FTA card-based nucleic acid purification was shown to rapidly detect\u00a0<em>E. coli<\/em>\u00a0O157:H7 with sensitivity and specificity. This visual method is expected to be widely used to control\u00a0<em>E. coli<\/em>\u00a0O157:H7 infections, particularly in resource-limited settings.<\/p>\n<p><a href=\"https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2023-0022\">https:\/\/www.scienceopen.com\/hosted-document?doi=10.15212\/ZOONOSES-2023-0022<\/a><\/p>\n<p><em>Zoonoses<\/em> is fully open access journal for research scientists, physicians, veterinarians, and public health professionals working on diverse disciplinaries of zoonotic diseases.<\/p>\n<p><em>Zoonoses<\/em> is now open for submissions; articles can be submitted online at <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a><\/p>\n<p>Please visit <a href=\"https:\/\/zoonoses-journal.org\/%20\">https:\/\/zoonoses-journal.org\/<\/a> to learn more about the journal.<\/p>\n<p><strong>Editorial Board:<\/strong> <a href=\"https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/\">https:\/\/zoonoses-journal.org\/index.php\/editorial-board\/<\/a><\/p>\n<p><strong><em>Zoonoses<\/em><\/strong> is available on <a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">ScienceOpen<\/a> (<a href=\"https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700\">https:\/\/www.scienceopen.com\/search#collection\/839df240-327f-47dd-b636-9b728dff9700<\/a>).<\/p>\n<p>Submissions may be made using <a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">ScholarOne<\/a> (<a href=\"https:\/\/mc04.manuscriptcentral.com\/zoonoses\">https:\/\/mc04.manuscriptcentral.com\/zoonoses<\/a>).<\/p>\n<p>There are no author submission or article processing fees.<\/p>\n<p>Follow <strong><em>Zoonoses <\/em><\/strong>on Twitter <a href=\"https:\/\/twitter.com\/ZoonosesJ\">@ZoonosesJ<\/a>; <a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">Facebook<\/a> (<a href=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\">https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114<\/a> ) and <a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">LinkedIn<\/a> (<a href=\"https:\/\/www.linkedin.com\/company\/zoonoses\/\">https:\/\/www.linkedin.com\/company\/zoonoses\/<\/a>)<\/p>\n<p><strong>eISSN <\/strong>2737-7474<\/p>\n<p><strong>ISSN<\/strong> 2737-7466<\/p>\n<p>Fumin Chen, Junyu Wang and Weiguang Li et al. Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification.\u00a0<em>Zoonoses.\u00a0<\/em>2023. Vol. 3(1). DOI: 10.15212\/ZOONOSES-2023-0022<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Announcing a new article publication for Zoonoses journal. Escherichia coli\u00a0O157:H7 (E. coli\u00a0O157:H7) can induce severe diseases in animals and humans that result in significant public health problems. Therefore, the development of rapid and visual detection methods to diagnose\u00a0E. coli\u00a0O157:H7 infections and monitor its prevalence is critical for the prevention and control purposes. A colorimetric loop-mediated [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[317,319,318,320],"class_list":["post-1104","post","type-post","status-publish","format-standard","hentry","category-news-and-events","tag-escherichia-coli-o157h7","tag-flinders-technology-associates-fta-card","tag-loop-mediated-isothermal-amplification-lamp","tag-molecular-diagnosis"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification - Zoonoses Journal<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/zoonoses-journal.org\/index.php\/2023\/09\/07\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification - Zoonoses Journal\" \/>\n<meta property=\"og:description\" content=\"Announcing a new article publication for Zoonoses journal. Escherichia coli\u00a0O157:H7 (E. coli\u00a0O157:H7) can induce severe diseases in animals and humans that result in significant public health problems. Therefore, the development of rapid and visual detection methods to diagnose\u00a0E. coli\u00a0O157:H7 infections and monitor its prevalence is critical for the prevention and control purposes. A colorimetric loop-mediated [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/zoonoses-journal.org\/index.php\/2023\/09\/07\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\/\" \/>\n<meta property=\"og:site_name\" content=\"Zoonoses Journal\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/Zoonoses-Journal-100462755574114\" \/>\n<meta property=\"article:published_time\" content=\"2023-09-07T05:32:13+00:00\" \/>\n<meta name=\"author\" content=\"Pdrtiru\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:site\" content=\"@ZoonosesJ\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Pdrtiru\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/07\\\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/07\\\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\\\/\"},\"author\":{\"name\":\"Pdrtiru\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#\\\/schema\\\/person\\\/7b94348b9f4f5d3983636ac0a201853f\"},\"headline\":\"Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification\",\"datePublished\":\"2023-09-07T05:32:13+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/07\\\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\\\/\"},\"wordCount\":371,\"publisher\":{\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/#organization\"},\"keywords\":[\"Escherichia coli O157:H7\",\"Flinders Technology Associates (FTA) card\",\"loop-mediated isothermal amplification (LAMP)\",\"molecular diagnosis\"],\"articleSection\":[\"News and Events\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/07\\\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\\\/\",\"url\":\"https:\\\/\\\/zoonoses-journal.org\\\/index.php\\\/2023\\\/09\\\/07\\\/visual-and-rapid-detection-of-escherichia-coli-o157h7-in-stool-samples-by-fta-card-based-loop-mediated-isothermal-amplification\\\/\",\"name\":\"Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification - 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